{"id":222,"date":"2024-11-06T14:35:00","date_gmt":"2024-11-06T05:35:00","guid":{"rendered":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/?page_id=222"},"modified":"2024-11-07T16:25:35","modified_gmt":"2024-11-07T07:25:35","slug":"keio-collection","status":"publish","type":"page","link":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/keio-collection\/","title":{"rendered":"Keio Collection"},"content":{"rendered":"\n<p> Details <br>\u30fbAs an E. coli gene <span style=\"color:#cf2e2e\" class=\"tadv-color\">knockout<\/span> strain, it was constructed by replacing the target gene with a kanamycin resistance gene fragment through homologous recombination. (PubMed:16738554)<br>\u30fb7,658 strains\uff083,829 non-essential genes, 2 strains each\uff09<br>\u30fbGenetically modified organism<\/p>\n\n\n\n<figure class=\"wp-block-image size-large is-resized\"><img loading=\"lazy\" src=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12-1024x488.png\" alt=\"\" class=\"wp-image-223\" width=\"586\" height=\"279\" srcset=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12-1024x488.png 1024w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12-300x143.png 300w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12-768x366.png 768w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12-670x320.png 670w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-12.png 1063w\" sizes=\"(max-width: 586px) 100vw, 586px\" \/><\/figure>\n\n\n\n<p>Utility<br>Comprehensive analysis of gene function<br>1. Drug susceptibility\/resistance<br>2. Heat tolerance<br>3. Metabolism, expression<br><\/p>\n","protected":false},"excerpt":{"rendered":"<p>Details \u30fbAs an E. coli gene knockout strain, it was constructed by replacing the target gene with a kanamycin  [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/222"}],"collection":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/comments?post=222"}],"version-history":[{"count":5,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/222\/revisions"}],"predecessor-version":[{"id":306,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/222\/revisions\/306"}],"wp:attachment":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/media?parent=222"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}