{"id":225,"date":"2024-11-06T14:36:57","date_gmt":"2024-11-06T05:36:57","guid":{"rendered":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/?page_id=225"},"modified":"2024-11-07T16:24:48","modified_gmt":"2024-11-07T07:24:48","slug":"aska-clone","status":"publish","type":"page","link":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/aska-clone\/","title":{"rendered":"ASKA Clone"},"content":{"rendered":"\n<p> Details <br>\u30fbA high-copy plasmid using the pCA24N vector (PubMed:16769691), in which the region encoding from the second to the last amino acid of the predicted E. coli gene was cloned.<br>\u30fb<span style=\"color:#cf2e2e\" class=\"tadv-color\">Expression<\/span> is inducible with IPTG<br>\u30fb3,900 strains<br>\u30fbGFP can be selected as an option<br>\u30fbGFP(+) is Genetically modified organism<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-13.png\" alt=\"\" class=\"wp-image-226\" width=\"572\" height=\"226\" srcset=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-13.png 905w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-13-300x119.png 300w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-13-768x304.png 768w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-13-670x265.png 670w\" sizes=\"(max-width: 572px) 100vw, 572px\" \/><\/figure>\n\n\n\n<p>Utility<br>Research utilizing high-copy plasmids<br>1. Construction of overexpression and complementation strains<br>2. Observation of intracellular localization<br>3. Purification of proteins using a His tag<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Details \u30fbA high-copy plasmid using the pCA24N vector (PubMed:16769691), in which the region encoding from the  [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/225"}],"collection":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/comments?post=225"}],"version-history":[{"count":4,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/225\/revisions"}],"predecessor-version":[{"id":304,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/225\/revisions\/304"}],"wp:attachment":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/media?parent=225"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}