{"id":231,"date":"2024-11-06T14:39:25","date_gmt":"2024-11-06T05:39:25","guid":{"rendered":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/?page_id=231"},"modified":"2024-11-07T16:27:01","modified_gmt":"2024-11-07T07:27:01","slug":"transbac-library","status":"publish","type":"page","link":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/transbac-library\/","title":{"rendered":"TransBac library"},"content":{"rendered":"\n<p> Details <br>\u30fbFrom start codon to stop codon<br>\u30fbLow-copy plasmid<br>\u30fb<span style=\"color:#cf2e2e\" class=\"tadv-color\">Expression<\/span> is inducible with IPTG<br>\u30fb3,462 strains<br>\u30fbPlasmid transfer via conjugation using the oriT of the F plasmid<br>\u30fbGenetically modified organism<\/p>\n\n\n\n<figure class=\"wp-block-image size-full is-resized\"><img loading=\"lazy\" src=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-15.png\" alt=\"\" class=\"wp-image-232\" width=\"571\" height=\"340\" srcset=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-15.png 731w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-15-300x179.png 300w, https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-content\/uploads\/sites\/2\/2024\/11\/image-15-670x399.png 670w\" sizes=\"(max-width: 571px) 100vw, 571px\" \/><\/figure>\n\n\n\n<p>Utility<br>Low copy number, allowing for expression close to natural levels<br> \u21d2 Useful for studying essential genes<br>Addressing potential issues with ASKA clones<br>\u3000Problems caused by the addition of a His tag<br>\u3000Issues during transformation<\/p>\n","protected":false},"excerpt":{"rendered":"<p>Details \u30fbFrom start codon to stop codon\u30fbLow-copy plasmid\u30fbExpression is inducible with IPTG\u30fb3,462 strains\u30fbPlasm [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":0,"menu_order":0,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/231"}],"collection":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/comments?post=231"}],"version-history":[{"count":3,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/231\/revisions"}],"predecessor-version":[{"id":307,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/231\/revisions\/307"}],"wp:attachment":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/media?parent=231"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}