{"id":238,"date":"2024-11-06T13:55:21","date_gmt":"2024-11-06T04:55:21","guid":{"rendered":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/?page_id=238"},"modified":"2025-07-30T10:13:27","modified_gmt":"2025-07-30T01:13:27","slug":"escherichia-coli-library","status":"publish","type":"page","link":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/our-microbial-strains-and-their-distribution\/escherichia-coli-library\/","title":{"rendered":"Escherichia coli Library"},"content":{"rendered":"\n

The Center has received permission from the rights holder, Nara Institute of Science and Technology (NAIST) (Developer: Dr. Hirotada Mori) to distribute the following collection. The collection can be used for comprehensive research on Escherichia coli, a model prokaryote organism.<\/p>\n\n\n\n

\u2460 Keio Collection<\/a>\u203b1
a set of single-gene deletion<\/span> mutants<\/p>\n\n\n\n

\u2461 ASKA Clone<\/a> GFP(+)\u203b1
a set of ORFs cloned  into the high copy<\/span> <\/strong>number plasmid with GFP<\/span><\/p>\n\n\n\n

\u2462 ASKA Clone<\/a> GFP(-)\u203b1
a set of ORFs cloned  into the high copy<\/span> number plasmid without GFP<\/span><\/p>\n\n\n\n

\u2463 Gateway Entry Clone library<\/a>\u203b1
a set of clone library for transfer<\/span> of the ORFs to other vectors<\/p>\n\n\n\n

\u2464 TransBac library<\/a>
a set of ORFs cloned  into the low copy<\/span> number plasmid<\/p>\n\n\n\n

\u2465 ASKA barcode deletion Collection<\/a> \u203b2
a set of single-gene deletion<\/span> mutants with barcode sequence<\/span><\/p>\n\n\n\n

\u2466 ASKA barcode deletion Collection<\/a> with Sm resistance \u203b2
a set of single-gene deletion<\/span> mutants with barcode sequence<\/span> and streptomycin<\/span> resistance<\/p>\n\n\n\n

\u203b1 : For the use of \u2460 – \u2463 in academic research at non-profit institutions, please contact NBRP E. coli Strain<\/a>.
\u203b2 : ASKA barcode deletion Collection will be available only for academic reseach at non-profit organizations.<\/p>\n\n\n\n

\u3000\u3000<\/p>\n","protected":false},"excerpt":{"rendered":"

The Center has received permission from the rights holder, Nara Institute of Science and Technology (NAIST) (D […]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":251,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238"}],"collection":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/comments?post=238"}],"version-history":[{"count":19,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238\/revisions"}],"predecessor-version":[{"id":364,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238\/revisions\/364"}],"up":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/251"}],"wp:attachment":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/media?parent=238"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}