{"id":238,"date":"2024-11-06T13:55:21","date_gmt":"2024-11-06T04:55:21","guid":{"rendered":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/?page_id=238"},"modified":"2026-05-19T09:56:42","modified_gmt":"2026-05-19T00:56:42","slug":"escherichia-coli-library","status":"publish","type":"page","link":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/our-microbial-strains-and-their-distribution\/escherichia-coli-library\/","title":{"rendered":"Escherichia coli Library"},"content":{"rendered":"\n<p>The Center holds the following collections, which were transferred from the Nara Institute of Science and Technology (NAIST, Developer: Dr. Hirotada Mori). The collections can be used for comprehensive research on Escherichia coli, a model prokaryote organism.<\/p>\n\n\n\n<p>\u2460 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/keio-collection\/\">Keio Collection<\/a>\u203b1<br>     a set of single-gene <span style=\"color:#cf2e2e\" class=\"tadv-color\">deletion<\/span> mutants<\/p>\n\n\n\n<p>\u2461 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/aska-clone\/\">ASKA Clone<\/a> GFP(+)\u203b1<br>     a set of ORFs cloned&nbsp; into the <span style=\"color:#cf2e2e\" class=\"tadv-color\">high copy<\/span><strong> <\/strong>number plasmid <span style=\"text-decoration: underline\" class=\"underline\">with GFP<\/span><\/p>\n\n\n\n<p>\u2462 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/aska-clone\/\">ASKA Clone<\/a> GFP(-)\u203b1<br>     a set of ORFs cloned&nbsp; into the <span style=\"color:#cf2e2e\" class=\"tadv-color\">high copy<\/span> number plasmid <span style=\"text-decoration: underline\" class=\"underline\">without GFP<\/span><\/p>\n\n\n\n<p>\u2463 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/gateway-entry-clone-library\/\">Gateway Entry Clone library<\/a>\u203b1<br>     a set of clone library for <span style=\"color:#cf2e2e\" class=\"tadv-color\">transfer<\/span> of the ORFs to other vectors<\/p>\n\n\n\n<p>\u2464 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/transbac-library\/\">TransBac library<\/a><br>      a set of ORFs cloned&nbsp; into the <span style=\"color:#cf2e2e\" class=\"tadv-color\">low copy<\/span> number plasmid<\/p>\n\n\n\n<p>\u2465 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/aska-barcode-delection-collection\/\">ASKA barcode deletion Collection<\/a> <br>     a set of single-gene <span style=\"color:#cf2e2e\" class=\"tadv-color\">deletion<\/span> mutants with <span style=\"text-decoration: underline\" class=\"underline\">barcode sequence<\/span><\/p>\n\n\n\n<p>\u2466 <a href=\"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/aska-barcode-delection-collection\/\">ASKA barcode deletion Collection<\/a> with Sm resistance<br>     a set of single-gene <span style=\"color:#cf2e2e\" class=\"tadv-color\">deletion<\/span> mutants with <span style=\"text-decoration: underline\" class=\"underline\">barcode sequence<\/span> and <span style=\"text-decoration: underline\" class=\"underline\">streptomycin<\/span> resistance<\/p>\n\n\n\n<p>\u203b1 : For the use of \u2460 &#8211; \u2463 in academic research at non-profit institutions, please contact <a href=\"https:\/\/shigen.nig.ac.jp\/ecoli\/strain\/locale\/change?lang=en\">NBRP E. coli Strain<\/a>.<br><\/p>\n\n\n\n<p>\u3000\u3000<\/p>\n","protected":false},"excerpt":{"rendered":"<p>The Center holds the following collections, which were transferred from the Nara Institute of Science and Tech [&hellip;]<\/p>\n","protected":false},"author":4,"featured_media":0,"parent":251,"menu_order":2,"comment_status":"closed","ping_status":"closed","template":"","meta":[],"_links":{"self":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238"}],"collection":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages"}],"about":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/types\/page"}],"author":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/users\/4"}],"replies":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/comments?post=238"}],"version-history":[{"count":21,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238\/revisions"}],"predecessor-version":[{"id":377,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/238\/revisions\/377"}],"up":[{"embeddable":true,"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/pages\/251"}],"wp:attachment":[{"href":"https:\/\/ds27i1.cc.yamaguchi-u.ac.jp\/~yurctmr1\/en\/wp-json\/wp\/v2\/media?parent=238"}],"curies":[{"name":"wp","href":"https:\/\/api.w.org\/{rel}","templated":true}]}}