Details
・A high-copy plasmid using the pCA24N vector (PubMed:16769691), in which the region encoding from the second to the last amino acid of the predicted E. coli gene was cloned.
・Expression is inducible with IPTG
・3,900 strains
・GFP can be selected as an option
・GFP(+) is Genetically modified organism

Utility
Research utilizing high-copy plasmids
1. Construction of overexpression and complementation strains
2. Observation of intracellular localization
3. Purification of proteins using a His tag