The Center has received permission from the rights holder, Nara Institute of Science and Technology (NAIST) (Developer: Dr. Hirotada Mori) to distribute the following collection. The collection can be used for comprehensive research on Escherichia coli, a model prokaryote organism.

① Keio Collection※
a set of single-gene deletion mutants

② ASKA Clone GFP(+)※
a set of ORFs cloned  into the high copy number plasmid with GFP

③ ASKA Clone GFP(-)※
a set of ORFs cloned  into the high copy number plasmid without GFP

④ Gateway Entry Clone library※
a set of clone library for transfer of the ORFs to other vectors

⑤ TransBac library
a set of ORFs cloned  into the low copy number plasmid

⑥ ASKA barcode deletion Collection
a set of single-gene deletion mutants with barcode sequence

⑦ ASKA barcode deletion Collection with Sm resistance
a set of single-gene deletion mutants with barcode sequence and streptomycin resistance

※For the use of ① – ④ in academic research at non-profit institutions, please contact NBRP E. coli Strain.