The Center has received permission from the rights holder, Nara Institute of Science and Technology (NAIST) (Developer: Dr. Hirotada Mori) to distribute the following collection. The collection can be used for comprehensive research on Escherichia coli, a model prokaryote organism.

① Keio Collection※1
a set of single-gene deletion mutants

② ASKA Clone GFP(+)※1
a set of ORFs cloned  into the high copy number plasmid with GFP

③ ASKA Clone GFP(-)※1
a set of ORFs cloned  into the high copy number plasmid without GFP

④ Gateway Entry Clone library※1
a set of clone library for transfer of the ORFs to other vectors

⑤ TransBac library
a set of ORFs cloned  into the low copy number plasmid

⑥ ASKA barcode deletion Collection ※2
a set of single-gene deletion mutants with barcode sequence

⑦ ASKA barcode deletion Collection with Sm resistance ※2
a set of single-gene deletion mutants with barcode sequence and streptomycin resistance

※1 : For the use of ① – ④ in academic research at non-profit institutions, please contact NBRP E. coli Strain.
※2 : ASKA barcode deletion Collection will be available only for academic reseach at non-profit organizations.