YU-RC for Thermotolerant Microbial Resources

Escherichia coli Library

The Center holds the following collections, which were transferred from the Nara Institute of Science and Technology (NAIST, Developer: Dr. Hirotada Mori). The collections can be used for comprehensive research on Escherichia coli, a model prokaryote organism.

Keio Collection※1
a set of single-gene deletion mutants

ASKA Clone GFP(+)※1
a set of ORFs cloned  into the high copy number plasmid with GFP

ASKA Clone GFP(-)※1
a set of ORFs cloned  into the high copy number plasmid without GFP

Gateway Entry Clone library※1
a set of clone library for transfer of the ORFs to other vectors

TransBac library
a set of ORFs cloned  into the low copy number plasmid

ASKA barcode deletion Collection
a set of single-gene deletion mutants with barcode sequence

ASKA barcode deletion Collection with Sm resistance
a set of single-gene deletion mutants with barcode sequence and streptomycin resistance

※1 : For the use of ① – ④ in academic research at non-profit institutions, please contact NBRP E. coli Strain.

  

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