Escherichia coli Library
The Center has received permission from the rights holder, Nara Institute of Science and Technology (NAIST) (Developer: Dr. Hirotada Mori) to distribute the following collection. The collection can be used for comprehensive research on Escherichia coli, a model prokaryote organism.
① Keio Collection※1
a set of single-gene deletion mutants
② ASKA Clone GFP(+)※1
a set of ORFs cloned into the high copy number plasmid with GFP
③ ASKA Clone GFP(-)※1
a set of ORFs cloned into the high copy number plasmid without GFP
④ Gateway Entry Clone library※1
a set of clone library for transfer of the ORFs to other vectors
⑤ TransBac library
a set of ORFs cloned into the low copy number plasmid
⑥ ASKA barcode deletion Collection ※2
a set of single-gene deletion mutants with barcode sequence
⑦ ASKA barcode deletion Collection with Sm resistance ※2
a set of single-gene deletion mutants with barcode sequence and streptomycin resistance
※1 : For the use of ① – ④ in academic research at non-profit institutions, please contact NBRP E. coli Strain.
※2 : ASKA barcode deletion Collection will be available only for academic reseach at non-profit organizations.