Details
・From start codon to stop codon
・Low-copy plasmid
・Expression is inducible with IPTG
・3,462 strains
・Plasmid transfer via conjugation using the oriT of the F plasmid
・Genetically modified organism

Utility
Low copy number, allowing for expression close to natural levels
⇒ Useful for studying essential genes
Addressing potential issues with ASKA clones
　Problems caused by the addition of a His tag
　Issues during transformation