Details
・As an E. coli gene knockout strain, it was constructed by replacing the target gene with a kanamycin resistance gene fragment through homologous recombination. (PubMed:16738554)
・7,658 strains（3,829 non-essential genes, 2 strains each）
・Genetically modified organism

Utility
Comprehensive analysis of gene function
1. Drug susceptibility/resistance
2. Heat tolerance
3. Metabolism, expression